多胺对水稻CMS系及其保持系幼穗蛋白质、核酸和活性氧代谢的影响

Effect of Polyamine on the Metabolism of Protein, DNA, RNA and Activated Oxygen in the Panicles of CMS Rice and Its Maintainer Line

用ＤＡｒｇ＋ ＭＧＢＧ 处理保持系， 降低花粉可育度， 并使其幼穗中蛋白质、ＤＮＡ 和ＲＮＡ含量以及蛋白酶、ＲＮＡ 酶和ＤＮＡ 酶活性下降，使Ｏ－·２ 生成速率和ＭＤＡ 含量上升。Ｐｕｔ＋ Ｓｐｄ ＋ Ｓｐｍ 可消除或部分消除ＤＡｒｇ ＋ＭＧＢＧ的上述效应（ 对酶活性的影响除外） 。ＤＡｒｇ ＋ ＭＧＢＧ 也使ＰＯＤ、ＳＯＤ 和ＣＡＴ活性上升， 但是，多胺只能降低抑制剂对ＰＯＤ 的刺激作用。用Ｐｕｔ＋ Ｓｐｄ ＋ Ｓｐｍ 处理不育系， 使花粉可育度轻度提高， 并使其幼穗蛋白质、ＤＮＡ和ＲＮＡ 含量略有上升，使蛋白酶、ＤＮＡ酶和ＲＮＡ 酶活性、Ｏ－·２ 生成速率、ＭＤＡ 含量、ＳＯＤ 和ＣＡＴ活性下降， 使ＰＯＤ

In CMS line, treatment with Put+Spd+Spm made pollen fertility (Table 1), and the contents of protein, DNA and RNA(Table 2) increased to some extent; but made the activities of protease, DNase and RNase decreased(Table 3). In the maintainer line, treatment with D Arg+MGBG made pollen fertility (Table 1) and contents of protein, DNA and RNA(Table 2), and the activities of protease, DNase and RNase(Table 3) decreased. Put+Spd+Spm, however, could partly counteract the effect of D Arg+MGBG on pollen fertility(Table 1) and contents(Table 2) of protein, DNA and RNA. After being treated with Put+Spd+Spm, the production rate of O -· 2, MDA content and the activities of SOD and CAT in CMS line decreased(Tables 4, 5). At the same time, however, the activity of POD increased(Table 5). In the maintainer line, the production rate of O -· 2 and MDA content increased by treatment with D Arg+MGBG. Put+Spd+Spm could counteract this effect(Table 4). Also, after being treated with D Arg+MGBG, the activities of POD, SOD and CAT increased. Put+Spd+Spm, nevertheless, could merely decrease the stimulating effect of D Arg+MGBG on POD(Table 5). Meanwhile, pollen fertility in the maintainer line was decreased by D Arg+MGBG(Table 1).