2,4-D对甘薯体细胞胚胎发生的调控

2,4 D Regulated Somatic Embryogenesis of Sweet Potato

将来源于‘徐薯１８’叶片的胚性愈伤组织，接种在含有不同２，４Ｄ浓度的液体ＭＳ培养基中进行悬浮培养，悬浮细胞表现出不同的形态结构、分裂方式和发育途径：２，４Ｄ浓度为１ｍｇ／Ｌ时，细胞均等分裂，增殖迅速；不含２，４Ｄ时，细胞多进行不均等分裂，并发育成体细胞胚。不同２，４Ｄ浓度中培养的悬浮细胞，其胞外过氧化物同工酶谱及其随时间变化的方式有很大差异，并与细胞的生长。

Divergences were discovered in cell morphology, mode of cell division and passage of cell differentiation, when embryogenic calli induced from leaves of `Xushu 18' sweet potato were suspension_cultured in modified MS medium with different concentrations of 2,4 D. Cells grew quickly and divided equally in medium containing 1 mg/L 2,4 D. When 2,4 D was deleted, the cells divided unequally and developed into embryos. Extracellular peroxidase isozyme patterns changed greatly during suspension culture with different concentrations of 2,4 D and were closely related to cell development process.