不同水温游泳对小鼠行为、记忆和脂质过氧化影响差异的研究

STUDY OF DIFFERENT EFFECT ON THE BEHAVIOR MEMORY AND FATTY SUPEROXIDE IN MICE BY SWIMMING AT DIFFERENT TEMPERATURE

目的 :探讨不同强度应激对小鼠衰老改变影响的差异。方法 :用D 半乳糖进行衰老诱导的小鼠同步进行不同水温 (12℃、18℃、2 5℃ )游泳应激训练 ,连续六周后 ,进行开场行为、学习记忆能力、半脑MDA含量及海马SOD活性测定。结果 :D 半乳糖诱导组 (D)较对照组 (C)探究行为减少 ,获得性行为能力减退 (P <0 .0 1) ,且脑内脂褐素含量明显增加 (P <0 .0 5 ) ;18℃及 2 5℃水温应激训练有对抗D 半乳糖所致行为和学习记忆能力减退的作用 (P <0 .0 1) ,同时脑中抗氧化能力提高 (P <0 .0 5 ) ,而 12℃水温组学习记忆能力进一步减退 ,海马SOD活性进一步下降(P <0 .0 5 ) ,且各项行为能力及SOD活性与 18℃和 2 5℃组均有显著差异 (P <0 .0 1)。结论 :不同水温游泳应激对机体的影响具有双重性 ,适量的应激刺激可逆转机体的衰老改变 ,提高动物的学习记忆能力 ,而过强的应激刺激有加速衰老进程的倾向。两种应激对机体抗氧化能力的不同改变可能是引起这一差异的原因。

Aim:In order To study different effect on the Aging process by stress of different degree.Methots: We toke swimming stress at different temperature as stress of different degree. While aging model was made by D galactose ip 0.12 mg/g/d for six weeks, the mice swimmed for 15 min/d at different water temperature (12℃、18℃ and 25℃). The open field behavior,the ability of learning and memory and the content of SOD and MDA in the brains were measured.Results:The swimmimg stress at 18℃ and 25℃ could increase ambulation and improve ability of discrimination in the Y maze and memory in step down test compared to D galactose group. The content of MDA were less than D Group(P<0.01) and SOD were more than D group( P <0.05). but, swimming at 12℃ promoted Aging process and showed significant diffrence from swimming at 18℃ or 25℃.Conclution:Stress serve a dual purpose: proper stress sti mulus can increase the activity of antioxidase in brain and improve ability of learning and memory of aging mice. In contrast,excessive stress stimulation promotes Aging process.