型糖尿病患者红细胞膜中性糖、氨基糖及唾液酸含量的改变

Changes in Erythrocyte Membrane Contents of Neutral Sugars, Hexosamines and Sialic Acid in Patients with NIDDM

为阐明Ⅱ型糖尿病（ Ｎ Ｉ Ｄ Ｄ Ｍ ）患者红细胞膜的化学组成在非酶糖基化（ Ｎ Ｅ Ｇ）影响下发生的变化，采用毛细管气相色谱法和分光光度法测定了 ２０ 名正常人和 １９ 例Ⅱ型糖尿病患者红细胞膜的 ４ 种结合中性糖与 ２ 种氨基糖和唾液酸含量以及 ４ 种寡糖链上的末端中性糖和唾液酸含量．结果表明， Ｎ Ｉ Ｄ Ｄ Ｍ 患者红细胞膜几种结合单糖（ Ｇｌｃ， Ｆｕｃ， Ｇｌｃ Ａ， Ｇａｌ Ａ）与游离单糖（ Ｇｌｃ， Ｇａｌ， Ｍａｎ， Ｆｕｃ）含量以及唾液酸含量均较正常对照组明显降低（ Ｐ＜ ００１ 或 ００５）．据此推测，由于患者红细胞膜蛋白的重度糖基化导致某些膜结构蛋白的氧化损伤，细胞膜糖类含量的减少，可能是重度糖基化的继发后果．

To explore what characteristic changes happened in the chemical composition of erythrocyte membranes in patients with non insulin dependent diabetes mellitus(NIDDM) subsequent to nonenzymatic glycosylation(NEG),erythrocyte membrane contents of combined neutral sugars(4 kinds)and hexosamines (2 kinds),sialic acid as well as those of the terminal neutral sugar(4 kinds)and sialic acid on the oligosaccharide chains were estimated by capillary gas chromatography and spectrophotometry in 20 healthy subjects and 19 NIDDM patients respectively.The erythrocyte membrane contents of several combined monosaccharides (Glc,Fuc,GlcA,GalA)and free monosaccharides(Glc,Gal,Man and Fuc)as well as sialic acid in the patient group were found to be significantly lower than those of the control group ( P <0 05 or 0.01).It is suggested that high degree of NEG in erythrocyte membrane proteins leads to the oxidative damage of certain membrane cytoskeleton proteins.An attenuation of membrane monosaccharide contents is possibly the subsequence of severe NEG.