多形核白细胞上嘌呤受体的初步研究

Study of Purinoceptors on Polymorphonuclear Leukocytes.

Ａ Ｔ Ｐ和 Ａ Ｄ Ｐ 能激活多型核白细胞引起细胞内［ Ｃａ２ ＋ ］ｉ 的明显升高， Ａ Ｍ Ｐ 则无此作用． 多型核白细胞对 Ａ Ｔ Ｐ和 Ａ Ｄ Ｐ 具有不同的浓度依赖性． 当细胞外的钙离子被螯合后， Ａ Ｔ Ｐ 和 Ａ Ｄ Ｐ 仍能引起细胞内游离钙浓度的升高． 结果表明多形核白细胞存在着对 Ａ Ｔ Ｐ 和 Ａ Ｄ Ｐ敏感的 Ｐ２ 型嘌呤受体， 并且属于 Ｐ２ 型受体中的 Ｐ２ Ｙ

Ca 2+ ] i transients were induced in porcine polymorphonuclear leukocytes in response to ATP and ADP, while no evident change in [Ca 2+ ] i was observed after AMP addition. The cells showed varied dose dependency upon ATP and ADP. In the Ca 2+ free bath solution, ATP and ADP could still cause [Ca 2+ ] i elevation in the cells. The results showed that the P2 receptors are present on porcine polymorphonuclear leukocytes and should belong to the P2Y subclass of purinoceptor family.