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昆津病毒复制子——一种新型病毒载体 被引量：2

Kunjin virus replicon——a novel viral vector
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摘要 病毒复制子(Replicon)是指来源于病毒基因组的能够自主复制的RNA分子,保留了病毒非结构蛋白基因,而结构蛋白基因缺失或由外源基因替代。昆津病毒(Kunjun virus)为黄病毒科黄病毒属成员,其复制子具有表达效率高、细胞毒性低、遗传稳定等特点,在病毒基因组复制调控机制、外源蛋白表达、新型疫苗和基因治疗等领域得到了广泛应用。以下就昆津病毒复制子系统的构建、特性及应用方面的研究进展作一综述。 Viral replicon is a kind of self-replicating viral RNA sourced from viral genome,which contains viral non-structural genes that are critical for viral genome replication with structural proteins deleted or replaced by foreign genes.Kunjin virus is a member of the Flavivirida family,Flavivirus genus,and Kunjin virus replicon is the first and the clearly defined flavivirus replicon.Kunjun virus replicon has been regarded as an excellent viral vector on account of its high expression,lower cytotoxicity and genetic stability.These unique characteristics of kunjin virus replicons make them suitable for the study of viral genome replication,recombinant proteins production,vaccine development and gene therapy.In this article,recent progress in the development,properties and applications of kunjin virus replicon system was briefly reviewed.
作者 李世华 李晓峰 秦鄂德 秦成峰
机构地区 军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室
出处 《生物工程学报》 CAS CSCD 北大核心 2011年第2期141-146,共6页 Chinese Journal of Biotechnology
基金 国家"十一五"重大科技专项课题(No.2008ZX10004-015)资助~~
关键词 昆津病毒 复制子 RNA病毒 病毒载体 Kunjin virus replicon RNA virus viral vector
分类号 Q939.4 [生物学—微生物学]
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参考文献22

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同被引文献25

  • 1俞永新.流行性乙型脑炎减毒活疫苗的发展和应用[J].上海预防医学,2006,18(3):110-112. 被引量：30
  • 2余云舟,俞炜源.黄病毒属病毒复制子载体研究进展[J].生物技术通讯,2006,17(2):228-231. 被引量：2
  • 3黄莺,邵炜,贾丽丽,俞炜源,俞永新.日本脑炎病毒(JEV)复制子表达载体的构建及其鉴定[J].病毒学报,2007,23(1):33-38. 被引量：6
  • 4Ryan L. Vander Veen,Alan T. Loynachan,Mark A. Mogler,Brandon J. Russell,D.L. Hank Harris,Kurt I. Kamrud.Safety, immunogenicity, and efficacy of an alphavirus replicon-based swine influenza virus hemagglutinin vaccine[J]. Vaccine . 2012 (11)
  • 5Zhenhua Yang,Rui Wu,Robert W. Li,Ling Li,Zhongliang Xiong,Haizhong Zhao,Deyin Guo,Zishu Pan.Chimeric classical swine fever (CSF)-Japanese encephalitis (JE) viral replicon as a non-transmissible vaccine candidate against CSF and JE infections[J]. Virus Research . 2012 (1)
  • 6Salini Krishnan Unni,Daniel R??ek,Chintan Chhatbar,Ritu Mishra,Manish K. Johri,Sunit K. Singh.Japanese encephalitis virus: from genome to infectome[J]. Microbes and Infection . 2011 (4)
  • 7Ying Huang,Shan Liu,Peng Yang,Chao Wang,Yun Du,Weiyuan Yu,Zhiwei Sun.Replicon-based Japanese encephalitis virus vaccines elicit immune response in mice[J]. Journal of Virological Methods . 2011 (1)
  • 8Fei Cao,Xiao-Feng Li,Xue-Dong Yu,Yong-Qiang Deng,Tao Jiang,Qing-Yu Zhu,E-De Qin,Cheng-Feng Qin.A DNA-based West Nile virus replicon elicits humoral and cellular immune responses in mice[J]. Journal of Virological Methods . 2011 (1)
  • 9F. Yu,F. Hasebe,S. Inoue,E. G. M. Mathenge,K. Morita.Identification and characterization of RNA-dependent RNA polymerase activity in recombinant Japanese encephalitis virus NS5 protein[J]. Archives of Virology . 2007 (10)
  • 10Peter W. Mason,Alexandr V. Shustov,Ilya Frolov.Production and characterization of vaccines based on flaviviruses defective in replication[J]. Virology . 2006 (2)

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生物工程学报
2011年 第2期

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