一种SDS-PAGE与MALDI-TOF质谱联用的方法

A Method of Recovering Protein from Dodecyl Sulfate polyacrylamide Gel for Matrix assisted Laser Desorption/Ionization Mass Spectrometry.

以尿激酶原为材料， 探索一种从 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 胶上回收蛋白质做 Ｍ Ａ Ｌ Ｄ Ｉ Ｔ Ｏ Ｆ 质谱的方法． 所用的回收方法包括电洗脱、脱盐、除 Ｓ Ｄ Ｓ 等过程． 电洗脱用的是高盐阻断法， 脱盐用的是超滤技术， 去除 Ｓ Ｄ Ｓ 用的是冷丙酮沉淀法． 结果证明， 此方法至少对一些蛋白质 （ 如尿激酶原和牛血清白蛋白）

The purpose of the experiment is to use pro urokinase as a model to find a way of recovering proteins from SDS PAGE gel for MALDI TOF MS. The recovering method includes steps of electroelution, desalting and SDS removal. Discontinuous conductivity gradient method for electroelution, centrifugation ultrofiltration for desalting and cold acetone method was used for SDS removal. The result shows that the method is feasible at least for some proteins (e.g.pro urokinase and bovine serum albumin ).