摘要
探讨了电转化过程中一些影响平连载体DNA转化效率的因素,并与化学法进行了比较,由此建立了优化的电转化条件。结果显示,在OD600值0.72-0.78收获细胞时,可使平连载体DNA的转化效率达到5×106转化子/μgcD-NA,较化学法高102-103倍。同时,降低连接产物的盐浓度。
A number of factors that infected transformation
efficiency of blunt-end ligated vector were investigated in this paper. By contrasting
transformation efficiency with chemical, an improved electrotransformation protocol was
establisheed. The results showed that the transformation efficiency of blunt-end ligated vector
can be up to 5x10 6 transformants from 1 μg cDNA when cells were harvested in OD 600 0.
72-0.78, which was in a 10 2-10 3 fold increase by comparing with chemical. In the same time,
it was important for the success of electrotransformation and the improvement of transformation
efficiency to lower salt concentration of ligation products.
出处
《生物技术》
CAS
CSCD
1999年第4期5-8,共4页
Biotechnology
基金
国家自然科学基金
中科院武汉分院测试基金资助项目
关键词
电转化法
感受态细胞
平连载体DNA
转化效率
electrotransformation,
competent cell, blunt-end ligated vector DNA transformation efficiency