摘要
用已经构建的工程菌高效表达抗人胃癌单链抗体，并使用ＳｅｐｈａｄｅｘＧ－１００柱层板分离纯化抗体蛋白。同时用免疫竞争抑制实验。
e have expressed efficiently ScFv antibody of antihuman stomach cancer in constructed
engineering strains. ScFv antibody were purified with SephadexG-100 gel-filtration
chromatography, its binding activities against the stomach carcinoma cells were detected by
immunological competing inhibition method.
出处
《生物技术》
CAS
CSCD
1999年第4期9-11,共3页
Biotechnology
基金
黑龙江省科委攻关项目
