用一种改进的电泳方法测定抗冻蛋白的分子量

TESTING OF MOLECULAR WEIGHT OF ANTIFREEZEPROTEIN BY AN IMPROVED ELECTROPHORESIS TECHNIQUE

针对抗冻蛋白分子量较小的特点，该文采用了一种改进的Ｔｒｉｃｉｎｅ－ＳＤＳ－ＰＡＧＥ法测定其分子量。采用三层胶系统并在电极缓冲液中以Ｔｒｉｃｉｎｅ代替Ｇｌｙｃｉｎｅ。

An improved tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis (Tricine-SDS-PAGE) technique was used to test the molecular weight of AFPs. A three-layer gel system was used and the glycine was insteaded by tricine in the buffer.