摘要
该文对原来的纤溶板法进行了改进，使测定结果更可靠，精确，并节省成本。用该法对ＣＬ－１１Ｇ工程细胞表达尿激酶原水平的稳定性观察表明，经１０４代连续传代，其表达量终始保持在５５９．６±９６．３１ＩＵ／１０６细胞／天左右，符合生产细胞的要求。
By means of comparing thhe diameter
of lysis zone of different dilutions of urokinase standard reference after different incubation
times, the originary fibrin-agarose-plate assay was slightly modificatied. The results were more
reliable, exact and the cose was more economic. With this modified assay we observed the
stability of prourokinase expressed by genetically-engineered cell line CL-11G. It showed that
the expression level of prourokinase was stable at 559.6±96.31IU/10 6 cells/day all the time
during 104 passages, so the CL-11G cell line was fitted the demand for production.
出处
《生物技术》
CAS
CSCD
1999年第4期42-46,共5页
Biotechnology
