儿茶素对血管平滑肌细胞的抑制作用及其可能的机制

Inhibitory effects and possible mechanisms of catechin on vascular smooth muscle cells

目的:探讨儿茶素对血管平滑肌细胞生长的抑制作用及其可能的作用机制。方法:采用MTT法检测不同剂量儿茶素对血管平滑肌细胞的抑制作用,分别用50、100、150、200、250、300μg/ml的儿茶素作用血管平滑肌细胞24和48 h后,观察细胞形态学的改变,并用RT-PCR检测儿茶素对血管平滑肌细胞基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)和基质金属蛋白酶抑制因子(tissue inhibitor of matrix metalloproteinase-1,TIMP-1)mRNA表达的影响。结果:250μg/ml儿茶素明显抑制细胞的生长,且明显下调血管平滑肌细胞中MMP-2 mRNA的表达,并同时上调TIMP-1 mRNA的表达。结论:儿茶素可以抑制血管平滑肌细胞的生长并且调节MMP-2和TIMP-1 mRNA的表达水平。

OBJECTIVE：To explore the inhibitory effects of catechin on vascular smooth muscle cells （VSMCs）and its possible mechanisms.METHODS：After VSMCs were exposed to different doses of catechin at 50,100, 150,200,250,300μg/ml,for 24 h and 48 h cell morphology was observed.MTT assay was used to test cell viability, mRNA expressions of MMP-2 and TIMP-1 were detected by RT-PCR analysis.RESULTS：Cell growth was inhibited after exposure to 250μg/ml,and mRNA expression MMP-2 was down-regulated.Meanwhile mRNA expression of TIMP-1 was up-regulated.CONCLUSION：Catechin could inhibit VSMCs and regulate mRNA expressions of MMP-2 and TIMP-1.