P63、Calponin和D2-40在涎腺肿瘤肌上皮细胞中的表达及意义

Expressions and clinical significance of P63,Calponin and D2-40 in myoepithelial cells of salivary gland tumors

目的:分析P63、Calponin、D2-40在各型涎腺肿瘤肌上皮细胞中的表达情况,探讨D2-40标记涎腺肿瘤肌上皮细胞的价值。方法:收集附属弋矶山医院2004年1月至2010年1月的6年间,手术切除的各型涎腺肿瘤标本切片作为研究材料。采用免疫组化SP法检测各型涎腺肿瘤组织中P63、Calponin、D2-40的表达。结果:P63、Calponin、D2-40均表达于肌上皮细胞,在各型涎腺肿瘤中总的表达率分别为82.14%、76.79%、66.07%。D2-40在多形性腺瘤、腺样囊性癌、上皮-肌上皮癌均有表达,表达率分别为94.12%、61.90%、66.67%,在恶性多形性腺瘤中未见表达。D2-40在多形性腺瘤、涎腺恶性肿瘤的癌旁组织、癌组织中均出现阳性表达,并以此为序其阳性表达率呈递减趋势,且3者间表达率的差异具有统计学意义(P<0.05)。结论:D2-40是一种较好的涎腺肿瘤肌上皮细胞的标记物;D2-40的低水平表达可能是涎腺肿瘤恶化的活跃指标之一。

OBJECTIVE：To analyze the expressions of P63,Calponin and D2-40 in myoepithelial cells of salivary gland tumors and to investigate the value of D2-40 in identification of myoepithelial cells in salivary gland tumor. METHODS：Various types of salivary gland tumor specimens were collected during surgeries in affiliated Yijishan Hospital from January 2004 to January 2010.We used immunohistochemical technique（S-P） to examine the expressions of P63,Calponin and D2-40 in each salivary gland tumor organization.RESULTS：P63,Calponin,D2-40 were expressed in myoepithelial cells,and the expressions of P63,Calponin,D2-40 were 82.14%,76.79%and 66.07%,respectively.D2-40 was expressed in polymorphic adenomas,adenoid cystic carcinoma,epithelial-myoepithelial carcinoma and the expressions were 94.12%,61.90%and 66.67%,respectively.D2-40 was not expressed in malignant polymorphic adenomas.The expression of D2-40 in polymorphic adenomas,tissues adjacent to the salivary gland malignancy and the cancerous tissues showed decreasing expressions that were significant（P0.05）.CONCLUSION：D2-40 could be one kind of ideal salivary gland tumor myoepithelial cell marker.Low expression of D2-40 is probably an indicator of malignant progresion of salivary gland tumor.