芩丹扶正胶囊对微波辐射致大鼠心肌和肝损伤超微结构的影响

Effect of Qindan Fuzheng Capsule on Ultrastructure of Microwave Radiation Injured Cardiomyocytes and Hepatocytes in Rats

目的探讨芩丹扶正胶囊对微波辐射致大鼠心肌和肝脏损伤超微结构的影响。方法 18只成年Wistar大鼠随机分为正常对照组、微波辐射组和微波辐射加药物治疗组,每组6只。正常对照组不予任何处理;微波辐射组采用微波辐射装置辐射6min,照射平均功率密度为100mW/cm2;微波辐射加药物治疗组照射条件同微波辐射组,微波辐射后6h开始用芩丹扶正胶囊灌胃,每次2mL(相当于4.75g生药),每天1次,连续7天。7天后处死各组大鼠,冰浴下立即取心脏心尖部和肝右叶新鲜组织,制作常规透射电镜标本并观察。结果与正常对照组比较,微波辐射组的心肌和肝细胞的细胞核与细胞器均有不同程度损伤;与微波辐射组比较,芩丹扶正胶囊治疗组的心肌和肝细胞体积正常,细胞核和细胞器保持良好,接近正常对照组细胞的形态。结论芩丹扶正胶囊对微波辐射致大鼠心肌和肝超微结构损伤有保护作用,其机制可能与抑制脂质过氧化、改善心肌和肝细胞的代谢有关。

Objective To explore effect of Qindan Fuzheng Capsule （QFC） on ultrastructure of cardiomyocytes and hepatocytes injured by high microwave radiation in rats. Methods Eighteen adult Wistar rats were equally divided into 3 groups in random：rats in Group A were untreated as the normal control,rats in Group B received 6 min microwave radiation （100 mW/cm2 high power） to cause injury of cardiomyocytes and hepatocytes,and Group C received the same radiation but treated with QFC perfusion,2 mL （equivalent to 4.75 g crude drug） once a day,for 7 successive days,starting from 6 h after radiation. All rats were sacrificed 7 days later,their fresh tissue of heart apex and right lobe of liver were taken and prepared to routine transmission electron microscopy specimen for ultrastructural observation. Results Compared with Group A,different degrees of ultrastructural changes on nuclei and organelle were observed in Group B and C,but the injury in Group C was significantly milder than that in Group B,showing normal sized cells with good structure approximate to the morphology in Group A. Conclusions QFC showed protective effect on microwave radiation injured ultrastructural changes in rats＇ cardiomyocytes and hepatocyte. Its mechanism was possibly corelated with the suppression of lipid peroxidation and the improvement of metabolism in myocardial and hepatic cells.