几种虫生真菌附着胞的荧光显微及扫描电镜观察

FLUORESCENCE AND SCANNING ELECTRON MICROSCOPY STUDY ON APPRESORIUM MORPHOLOGY OF SOMEENTOMOGENOUS FUNGI

本研究通过对细脚拟青霉、蝉拟青霉、玫烟色拟青霉、金龟子绿僵菌和莱氏野村菌在疏水表面产生附着胞的荧光显微镜观察和扫描电镜观察，明确五种虫生真菌均可产生附着胞，细脚拟青霉和金龟子绿僵菌产生单附着胞和复合附着胞两种形态，均呈椭圆至长椭圆形，如遇到不适合浸染的部位，则重新产生芽管向前延伸直至找到适合入侵的部位。蝉拟青霉分生孢子多在顶端发芽成人字形，末端形成椭圆形附着胞，该拟青霉再生附着胞能力强。金龟子绿僵菌和莱氏野村菌附着胞表面有较厚的粘液层，而三种拟青霉附着胞表面均未见有粘液层。

By techniques of fluorescence microscopy and scanning electron microscopy,appresorium morphology of some entomogenous fungi, Paecilomyces tenuipes , P. cicadae, P. fumosoroseus,Metarhizium anisopliae and Nomuraea rileyi, was studied on hydrophobic hard surface. The results showed that all of the 5 species produced appresoria. Germ tubes of P. tennipes and M anisopliae conidia produced both unicellular and multicellular appresoria or regerminated to look for a proper invading place , Conidia of p.cicadae produced a long germ tube before formation of an appresorium, and was capable of reproducing appresorium. Surface of germ tUbes and appresoria of M anisopliae and N rileyi was covered by a thick layer of mucilage, but the mucilage layer was not obvious with the other 3 species.