绒毛膜癌细胞的融合与侵袭和转移能力变化关系的研究

The Study of the Relationship of Fusion Status of Choriocarcinoma Cells and Their Invasive or Metastatic Capability

目的:观察人绒毛膜癌(绒癌)细胞系中BeWo细胞株在福斯考林(forskolin)诱导融合前后细胞中基质金属蛋白酶-2(MMP-2)及上皮钙黏蛋白(E-cad)的表达变化,以探讨绒癌细胞融合与其浸润和转移能力变化的关系。方法:用BeWo细胞株进行培养传代,以100mmol/L浓度的forskolin作用于BeWo细胞,用逆转录聚合酶链(RT-PCR)方法检测细胞中人类内源性逆转录病毒基因编码的膜糖蛋白(Syncytin mRNA)表达量的变化;免疫印迹技术(Western blot)方法检测细胞中表达MMP-2及E-cad蛋白表达量的变化。结果:以BeWo细胞作为靶细胞,根据RT-PCR和Western blot方法中条带的形状和亮暗程度可知Syncytin基因和E-cad及MMP-2蛋白的表达呈时间依赖性,随着forskolin作用时间的增加,Syncytin基因及E-cad蛋白的表达逐渐增加,而MMP-2蛋白表达量逐渐减少,差异具有统计学意义(P<0.05)。结论:Syncytin高表达与融合后绒癌细胞的浸润转移能力的改变可能有一定关系,Syncytin的检测有望成为滋养细胞肿瘤患者判定预后的有价值的指标,并为滋养细胞肿瘤的治疗开辟了新的思路。

Objective：To observe the changes of the expressions of MMP-2 and E-cad before and after Forskolin treating the Human Choriocarcinoma BeWo cell line,to investigate the relationship of the fusion status of Choriocarcinoma cells and their invasive and metastatic capabiligy. Methods：BeWo cell lines were cultured and passaged,then were treated by 100mmol/L Forskolin. Syncytin mRNA was detected by RT-PCR,also the changes of the protein,MMP-2 and E-cad were checked by Western blot. Results：According to the shape and brightness of PCR and Western bands,Syncytin mRNA and E-cad,MMP-2 protein showed time-dependent changes. Along with the reaction of Forskolin,the expression of Syncytin mRNA and E-cad protein increased steadily,while the expression of MMP-2 protein decreased,the differences were significant （P〈0.05）.Conclusions：The high expression of syncytin might be related with the changes of invasive or metastatic capability post fusion of choriocarcinoma cells. So Syncytin might be a valueble prognostic marker and offer a new treatment ragimen for trophoblastic tumor.