卷曲霉素产生菌的改良

IMPROVEMENT OF CAPREOMYCIN-PRODUCING STREPTOMYCES CAPREOLUS

以卷曲霉素（ＣＰＭ）产生菌卷曲链霉菌Ｓ．ｃａｐｒｅｏｌｕｓ４００６（效价为６６４０ｕ／ｍＬ）为出发菌，经ＵＶ诱变／五氯酚浓缩，获得一株效价为７９５０ｕ／ｍＬ的营养缺陷型菌株４０６－３５，再经ＥＭＳ诱变，筛选到一株抗５０００ｕ／ｍＬＣＰＭ、效价为８５１３ｕ／ｍＬ的突变株５－４１，经原生质体ＮＴＧ诱变处理，获得一株效价为９０５０ｕ／ｍＬ的高产菌Ｎ１３菌株Ｎ１３连续传代１０代，卷曲霉素效价保持稳定通过单因子试验和多因子正交试验，确定了菌株Ｎ１３的最佳发酵条件菌株Ｎ１３在最佳发酵条件下卷曲霉素效价为９４８３ｕ／ｍＬ，比出发菌Ｓ．

Using capreomycin producing Streptomyces capreolus 4 006 (with the yield of capreomycin being 6640 u/mL) as parent strain, after UV mutation and eutrophication with pentachlorophenol, an auxotrophic strain 4006-35 producing 7 950 u/mL of capreomycin was obtained. Subsequently the auxotrophic strain 4006-35 was treated with EMS, and the mutant 5-41 resistant to 50 000 u/mL of CPM was obtained and the mutant produced 8 513 u/mL of CPM. After the protoplasts of strain 5-41 were treated with NTG, a mutant strain N13 producing 9 050 u/mL of capreomycin was obtained and strain N13 was stable in capreomycin production after continuous subcultures for 10 generations. Optimum fermentation conditions were established based on the study on fermentation conditions for strain N13. Under the optimum conditions, the capreomycin yield by N13 was 9483 u/mL and increased by 42.8% compared with the original parent strain 4006 under the original fermentation conditions.