摘要
采用聚乙烯醇(PVA)加少量海藻酸钠及活性碳的方法对一株能高效降解甲胺磷的细菌假单胞菌B82菌株进行固定化.正交试验结果表明,三者合适配比为聚乙烯醇100(ρ/gL-1),海藻酸钠5(ρ/gL-1),活性碳50(ρ/gL-1).此方法制得的凝胶颗粒机械强度好、经久耐用.固定化B82凝胶小球能在pH6~11的范围内发挥其降解甲胺磷的作用,其最适作用温度为35℃.B82菌经固定化后,降解甲胺磷的速度明显加快.
A strain from soil idendtified as Pseudomonas sp. (strain B 82), which can degrade methamidophos efficiently, was immobilized in the PVA H 3BO 3 gel beads with small amount of sodium alginate and activated carbon. The results of orthogonal tests showed that optimal concentrations of the three substances were 100( ρ /g L -1 ) PVA, 5( ρ /g L -1 ) sodium alginate and 50( ρ /g L -1 ) activated carbon. The gel beads were durable and had great mechanical strength. This strain could effectively degrade methamidophos with pH ranging 6 10 and optimal temperature 35℃. After it was immobilized, the strain was able to degrade methamidophos at a faster rate than free cells.
出处
《应用与环境生物学报》
CAS
CSCD
1999年第4期422-426,共5页
Chinese Journal of Applied and Environmental Biology