固定化假单胞菌降解甲胺磷的研究

DEGRADATION OF ORGANOPHOSPHATE PESTICIDE METHAMIDOPHOS BY IMMOBILIZED PSEUDOMONAS SP.

采用聚乙烯醇（ＰＶＡ）加少量海藻酸钠及活性碳的方法对一株能高效降解甲胺磷的细菌假单胞菌Ｂ８２菌株进行固定化．正交试验结果表明，三者合适配比为聚乙烯醇１００（ρ／ｇＬ－１），海藻酸钠５（ρ／ｇＬ－１），活性碳５０（ρ／ｇＬ－１）．此方法制得的凝胶颗粒机械强度好、经久耐用．固定化Ｂ８２凝胶小球能在ｐＨ６～１１的范围内发挥其降解甲胺磷的作用，其最适作用温度为３５℃．Ｂ８２菌经固定化后，降解甲胺磷的速度明显加快．

A strain from soil idendtified as Pseudomonas sp. (strain B 82), which can degrade methamidophos efficiently, was immobilized in the PVA H 3BO 3 gel beads with small amount of sodium alginate and activated carbon. The results of orthogonal tests showed that optimal concentrations of the three substances were 100( ρ /g L -1 ) PVA, 5( ρ /g L -1 ) sodium alginate and 50( ρ /g L -1 ) activated carbon. The gel beads were durable and had great mechanical strength. This strain could effectively degrade methamidophos with pH ranging 6 10 and optimal temperature 35℃. After it was immobilized, the strain was able to degrade methamidophos at a faster rate than free cells.