脆壁克鲁维酵母蛋白二硫化物异构酶KfPDI的结构分析

Structure Analysis on Protein Disulfide Isomerase Gene of Kluyveromyces fragilis

利用ＤＮＡ限制性内切酶图谱分析将一个带有脆壁克鲁维酵母 （Ｋｌｕｙｖｅｒｏｍｙｃｅｓｆｒａｇｉｌｉｓ） 蛋白二硫化物异构酶基因（ＫｆＰＤＩ） 的１０ ｋｂ 初始克隆缩短为４ ｋｂ 的亚克隆，并测定了该基因的ＤＮＡ全序列。经分析发现： （１）该基因编码产物由５２０个氨基酸残基组成。（２） 它与乳酸克鲁维酵母（Ｋ． ｌａｃｔｉｓ） 蛋白二硫化物异构酶在氨基酸序列上的同源性为７８。（３） 它与其他物种的ＰＤＩ基因在核苷酸和蛋白质水平上的同源性较弱，

A 10 kb of primary DNA clone containing the Kluyveromyces fragilis protein disulfide isomerase gene (KfPDI) was mapped and shortened into a 4 kb of subclone, followed by the complete gene DNA sequencing and analyzing. The results show that: (1) the KfPDI protein is consisted of 520 amino acid residues; (2) the homologous identity between KfPDI and the KlPDI from K. lactis is 78% at amino acid level; (3) Although the homology between KfPDI and other known PDI genes is not perfect but two highly conserved activity domains of protein disulfide isomerase can be found among all of them.