地衣芽孢杆菌在麸曲中产中性蛋白酶发酵工艺条件的研究

Study on the Fermentation Conditions of Bacillus Licheniformis in Bran Starter to Produce Neutral Protease

对地衣芽孢杆菌在细菌麸曲培养过程中的最佳培养基和最佳培养条件进行了研究。结果表明,地衣芽孢杆菌麸曲产中性蛋白酶的最佳培养基为:玉米粉8g/L,黄豆粉2g/L,麸皮10g/L;最佳培养条件为:温度40℃,pH7.0,接种量10%,培养60h时,产中性蛋白酶活力达到最大,为192U/mL。

In this study, the best culture medium and the best culture conditions of Bacillus licheniforrnis in the culture process of bacteria bran starter were investigated. The results showed that the best culture medium of Bacillus licheniformis to produce neutral protease was comprised of maize 8 g/L, bean flour 2 g/L, and bran 10 g/L, The best culture conditions were temperature at 40 ℃, pH=7.0, 10 % inoculating quantity, and 60 h culture time. Under the above conditions, the activity ofproduced neutral protease could reach the maximum as 192 U/mL.