分光光度法检测不同地区和类型稻种功能性成分的初步研究

A preliminary study on the functional ingredients in brown rice from different regions and types varieties

为研究不同地区和类型水稻品种糙米中γ-氨基丁酸和总黄酮等功能性成分,本实验采用分光光度法准确测定了中国、日本、韩国、国际稻作所和云南品种及云南野生稻γ-氨基丁酸和总黄酮的含量.结果表明:①用分光光度法测定水稻籽粒中γ-氨基丁酸和总黄酮含量的方法是可靠的;②不同地区和类型水稻品种功能成分含量存在差异;③相关分析表明,氨基丁酸与总黄酮的相关系数较高(r=0.575**),在育种上可以实现多个相关性状间的遗传改良;④鉴定出墨江黄皮饵块谷、明恢86、墨江饵块谷、疣粒野生稻等18份高GA-BA和高总黄酮的水稻品种,可作为选育高黄酮和γ-氨基丁酸品种的亲本或直接作为功能稻开发利用.首次报道疣粒野生稻总黄酮质量分数达0.751%,在所有水稻品种中黄酮含量最高.

Study on the contents of γ-aminobutyric acid and total flavones in brown rice of different type varieties from China,Japan and Korea is conducted by colorimetry,the varieties comprise IRRI,local varieties and wild rice of Yunnan.The main results are as follows：Colorimetry is a reliable method of determining the contents of γ-aminobutyric acid and total flavonoid,the contents of the functional ingredients show significant differencein different regions and types;There is significant correlation（r=0.575＊＊）between the contents of γ-aminobutyric acid and total flavones,and genetic improvement could be achieved with these related traits;Among all the tested varieties,18 accessions with high γ-aminobutyric acid and total flavonoid such as huangpierkuaigu,minghui86,erkuaigu,oryza meyerian of Yunnan are screened out and identified as good parents strains or even seed sources with high contents of functional ingredients.It is firstly reported that total flavonoids of wild rice was the highest as 0.751% in all varieties.