摘要
目的通过分析HBV DNA含量、Pre-S1蛋白及HBeAg之间的关系,探讨三者在反映乙肝病毒复制中的价值及临床意义。方法采用酶联免疫吸附试验(ELISA)检测HBsAg阳性标本的Pre-S1及HBe-Ag,酶标仪检测其吸光度值,实时荧光定量聚合酶联反应(PCR)检测HBV DNA含量。结果在HBV DNA阳性或阴性标本中,Pre-S1阳性检出率明显高于HBeAg(P=0.005),但Pre-S1的吸光度值在两者中无差异,而HBeAg在HBV DNA阳性标本中的吸光度(S/C0=21.167±9.597)高于在阴性标本中的值(S/C0=2.915±1.683),两者比较P=0.006;在HBeAg阳性标本中,HBV DNA和Pre-S1的阳性检出率无差异,而在阴性标本中Pre-S1的阳性检出率明显高于HBV DNA检出率,并且HBV DNA含量和Pre-S1吸光度值两者差异有统计学意义(P<0.05);在Pre-S1阳性标本中,HBV DNA阳性率高于HBeAg的阳性率,而且HBV DNA含量也高于在阴性标本中含量(P=0.022)。结论 HBV DNA含量、Pre-S1蛋白及HBeAg在血清中表达意义的侧重点可能不同,在实际工作中应联合检测,才能较全面地反映病毒在患者体内的真实情况,为临床治疗提供更加客观可靠的实验数据。
Objective To investigate the value of HBV DNA,PreS1 and HBeAg in reflecting HBV replication and clinical significance by analysing their relationship.Methods Enzyme linded immunosorbent assay（ELISA） was adopted to detect PreS1 protein,HBeAg,absorbance value was examined by Enzyme sign meter,and HBV DNA content was measured with real-time PCR in the cases of serum specimens with positive HBsAg.Results The positive rate of Pre-S1 was higher than HBeAg（P=0.005） whether in positive or negative serum specimens of HBV DNA,there was no difference in absorbance value of Pre-S1 between them,but the absorbance value of HBeAg was higher in positive specimens（S/C0=21.167±9.597） than in negative（S/C0=2.915±1.683,P=0.006））.The positive rate of HBV DNA and Pre-S1 was no difference in positive specimens of HBeAg,in negative serum of HBeAg,the positive rate of Pre-S1 was higher than HBV DNA,HBV DNA content and the absorbance value of Pre-S1 was different in positive or negative serum of HBeAg（P0.05）.In positive specimens of Pre-S1,the rate of HBV DNA was higher than HBeAg,the content of HBV DNA was higher than in negative specimens of Pre-S1（P=0.022）.Conclusion The clinica value of HBV DNA content,Pre-S1 protein and HBeAg express in serum is different,so combined detection should be performed to indicate the real condition of HBV in patients,and provide reliable empirical date for clinical treatment.
出处
《安徽医学》
2011年第2期143-145,共3页
Anhui Medical Journal
