摘要
鉴于有性世代形成能高的稻瘟病菌菌株有限,稻瘟病菌间的杂交组合难于获得,从而很难对一些重要基因,如无毒基因,抗药性基因等进行遗传分析及深入研究。本研究利用原生质体融合技术获得梨孢菌的杂合子,从而评价该体系用于遗传研究的可行性。首先,利用转基因技术获得稻瘟病菌Y93-164a-1跟蟋蟀草病菌SA98-4的抗药性转化子,然后将两个菌的稳定转化子的原生质体进行融合,之后进行连续5次的继代培养,最后进行3次单孢分离来挑选稳定的融合子。结果表明,利用原生质体融合技术在两个不同致病型的梨孢菌之间能获得稳定的融合子,初步表明利用原生质体融合技术组建梨孢菌的融合子群体用于遗传分析在技术上是可行的。
The genetic analysis of important genes,e.g.avirulence genes,antibiotic resistant genes in Magnaporthe oryzae pathogens had been limited because of the difficulty of getting highly fertile strains for genetic cross,especially in Oryza pathotype strains.In the present study,an alternative method for the genetic study of this fungus was tried by using the protoplast fusion system.Firstly,drug-resistant transformants were generated from the Oryza strain Y93-164a-1 and Eleusine pathotype strain SA98-4,then protoplasts of both selected transformants were fused by protoplast fusion technique.Stable fusants were selected after at least five subcultures and three single-spored isolations on the selective medium.Results suggested that the stable fusants can be easily obtained by protoplast fusion system,showing the protoplast fusion system could be usable for genetic analysis of M.oryzae pathogens.
出处
《西南农业学报》
CSCD
北大核心
2011年第1期233-239,共7页
Southwest China Journal of Agricultural Sciences
基金
supported by Program for New Century Excellent Talents in University(NCET-08-0783)
supported by a project (11660050) from the Ministry of Education,Culture,Sports,Science and Technology of Japan
the project of the Scientific Research Foundation for the Returned Overseas Chinese Scholars,Huazhong Agricultural University,China(4002-30541).
关键词
梨孢属
稻瘟病菌
原生质体融合
遗传分析
Magnaporthe oryzae
Rice blast fungus
Protoplast fusion
Genetic analysis
