润滑油降解菌的筛选及其alkB基因的PCR分析

Screening of Bacterial Strains for Degradation of Lubricating Oil and Their Analysis for Presence of Alkane Hydroxylase Gene (alkB) by PCR

从重庆地区受润滑油污染的酸性土壤中筛选润滑油降解菌,采用形态学和16 srDNA分析鉴定其分类地位,研究它们以润滑油作为唯一碳源的生长能力,并对其烷烃羟化酶基因(alkB)进行了PCR分析。从污染的土壤样品中分离出11株细菌。用酶法检测和干重法检测发现9株细菌均能分解利用润滑油,16 srDNA分析表明这些菌分属Pseudom onas,Cupriavidus,Bacillus,Acine-tobacter,Stenotrophom onas。对这些菌株的PCR分析发现其alkB基因扩增片段大小介于160和400 bp之间。

Lubricating oil degrading bacteria were isolated from polluted acidic soil in Chongqing.Their taxonomic status were investigated by morphology observation and alignment analysis of 16s rDNA.The isolated bacteria＇s growing characteristics were tested when growing on lubricating oil as the sole carbon source.Their alkane hydroxylase gene（alkB） were analyzed by PCR.As a result,11 strains of bacteria were isolated.By enzymatic assay and the dry weight assay,9 strains were found to have ability to degradate lubricating oil.16srDNA analysis showed that these strains respectively belonged to Pseudomonas,Cupriavidus,Bacillus,Acinetobacter,Stenotrophomonas.PCR analysis suggested the alkB gene in isolated bacteria had different fragment sizes with the range between 160 and 400 bp.