Fe_3O_4纳米粒子与细胞的相互作用研究

Investigation of Fe_3O_4 nanoparticles interacting with cells

研究10 nm粒径的表面未修饰Fe3O4纳米粒子在体外对人正常肝细胞HL-7702及人肝癌细胞SMMC-7721的生长影响。通过倒置显微镜、透射电镜(TEM)观察加入Fe3O4纳米粒子后肝癌细胞和正常肝细胞的形态变化及磁性纳米粒子在细胞内分布状态。用CCK-8测定加入磁性纳米粒子培养后细胞增殖能力的变化。倒置显微镜、透射电镜(TEM)结果表明:在实验浓度(200μg/mL)以下,Fe3O4纳米粒子作用未表现急性毒性。Fe3O4纳米粒子两种细胞72 h后,细胞形态均保持正常;Fe3O4纳米粒子能够进入细胞质,主要分布于吞噬溶酶体和吞噬小体。CCK-8检测Fe3O4纳米粒子对细胞的增殖无明显影响。此实验结果为磁性纳米粒子与肿瘤细胞微观结构的作用提供了有意义的实验数据,并对应用纳米粒子对人肝癌细胞进行胞内热疗提供指导。

The biological behavior of magnetic nanoparticles with diameter of 10 nm on human liver cancer line SMMC7721 and human normal liver cell line HL7702 in vitro was studied.Both cells were cultured in the media containing Fe3O4 nanoparticles.Inverted microscopy and transmission electron microscopy（TEM） were used to investigate the morphologies of cells and the nanoparticles distribution in the cells;CCK-8 assay was used to evaluate the effects of the nanoparticls on the proliferation of cells.According to inverted microscopy and TEM analysis,the nanoparticle-exposed cells are normal in morphologies after 72h.The nanoparticles can enter the cytoplasm and distribut in the phagosome and phagolysosome.The result of CCK-8 array show the naoparticle has no significant effect on cells proliferation.This study provides detailed data for the effect of magnetic nanoparticles on liver cancer cell.It also offers valuable data for the further application of magnetic nanoparticles in tumor hyperthermia.