p16和Ki67在宫颈病变中的临床意义

Expression of p16 and Ki67 in cervical intraepithelial neoplasia and cervical carcinoma

收集2007年1月至2009年6月在新疆维吾尔自治区克拉玛依市中心医院妇科体检妇女共9573例,发现细胞学异常274例,阴道镜活检提示正常宫颈组织111例,宫颈上皮内瘤变（CIN）153例,宫颈癌10例.采用免疫组化法检测274例标本中的p16、Ki67的表达情况.结果 示正常宫颈组织、CIN Ⅰ、CINⅡ、CINⅢ、宫颈癌的p16阳性率分别为9.0%、69.9%、93.8%、95.5%、10/10,Ki67阳性率分别为76.6%、89.2%、91.7%、95.5%、10/10.p16和Ki67的表达强度与宫颈病变的严重程度相关（r=0.681和r=0.314,均P＜0.01）.应用p16和Ki67免疫组化染色有助于宫颈病变诊断,可作为诊断的重要指标.

Two hundred and seventy four patients with suspicious cervical lesions underwent pelvic examination and biopsy. Tissue biopsy results revealed 153 cases of cervical intraepithelial neoplasia（CIN）and 10 cases of cervical carcinoma. p16 and Ki67 were detected by immunohistochemistry method in tissue specimens. The positive rates of p16 were 9.0%, 69. 9%, 93. 8%, 95. 5% and 10/10 in normal cervical mucosa, CIN Ⅰ , CIN Ⅱ , CIN Ⅲ and cervical carcinoma, respectively; while those of Ki67 were 76. 6%,89. 2%, 91.7%, 95.5% and 10/10, respectively. The expressions of p16 and Ki67 were significantly associated with cervical lesions （ P 〈 0. 01 ）. The study suggests that p16 and Ki67 may be used as supplemental diagnostic markers for cervical lesions.