老年糖尿病患者血清褪黑素水平和白细胞诱导型一氧化氮合酶mRNA的表达

Serum melatonin levels and expression of inducible nitric oxide synthase mRNA in leucocytes of elderly diabetic patients

对70例新诊断的糖尿病患者和30例老年健康者（对照组）,检测空腹血清褪黑素及外周血白细胞中诱导型一氧化氮合酶（iNOS）mRNA表达水平.老年起病的糖尿病患者（37例）的血清褪黑素水平明显低于对照组[（6.6±1.3）与（7.7±1.4）ng/L,P＜0.01];外周血白细胞iNOS mRNA表达水平明显高于成年起病的糖尿病患者[（36±3）%与（30±4）%,P＜0.01],对照组无iNOS mRNA表达.多元logistic回归分析显示,血清褪黑素水平下降及外周血白细胞中iNOS mRNA表达水平增高,可能是老年人发生糖尿病的危险因素.

Seventy newly diagnosed diabetic patients were enrolled in the study, including 37patients aged over 60 （elderly-onset diabetes, EOD group） and 33 patients under 60 （adult-onset diabetes,AOD group） ; 30 elderly healthy subjects served as controls. Serum melatonin levels were determined with ELISA and the expression of inducible nitric oxide synthase （iNOS） mRNA in peripheral blood leucocytes （PBL） was detected by reverse transcription polymerase chain reaction （RT-PCR）. The results showed that serum melatonin levels were significantly lower in EOD group than those in control group [ （6. 6 ± 1.3 ）ng/L vs. （7.7 ± 1.4） ng/L, P 〈0. 01 ], the expression of iNOS mRNA was significantly higher than that in AOD group [ （36 ± 3 ）% vs. （30 ± 4）%, P 〈 0. 01 ]; and i NOS mRNA was not detected in control group.Logistic regression analysis indicated that decreased serum melatonin levels and up-regulated iNOS mRNA might be independent risk factors for diabetes mellitus in the elderly.