摘要
目的:探讨巨噬细胞移动抑制因子(MIF)基因启动子区-794CATT微卫星多态性与子宫内膜异位症的遗传易感性的相关性。方法:应用聚合酶链反应(PCR)分析技术及DNA测序技术,检测广东籍汉族子宫内膜异位症患者43例(内异症组)及非子宫内膜异位症患者44例(对照组)MIF基因启动子区-794CATT微卫星多态性,并进行MIF基因分型。结果:①内异症组MIF基因启动子区-794CATT基因型(5/5,5/6,5/7,6/6,7/7)分布频率分别为51.2%、11.6%、4.7%、30.2%、2.3%;对照组(44例)MIF基因启动子区-794CATT基因型(5/5,5/6,5/7,6/6,6/7,7/7)的分布频率分别为25.0%、11.4%、2.3%、50.0%、4.5%、6.8%。MIF基因启动子区-794CATT(5/5)基因型的分布频率两组间比较差异具有统计学意义,内异症组高于对照组(P=0.015,OR=3.143,95%CI=1.281～7.713)。②MIF基因启动子区-794CATT(5,6,7)等位基因型在内异症组中的分布频率分别为59.3%、36.0%、4.7%;在对照组中分别为31.8%、58.0%、10.2%;MIF基因启动子区-794CATT(5)等位基因型两组间比较差异具有统计学意义,内异症组高于对照组(P=0.038,OR=3.122,95%CI=1.688～5.774)。结论:MIF基因启动子区-794CATT微卫星多态性与广东汉族人群子宫内膜异位症的遗传易感性相关,CATT(5/5)基因型及CATT(5)等位基因型可能是其易感基因型。
Objective：To explore the correlation between polymorphism of migration inhibitory factor（MIF） gene promoter region-794CATT microsatellite and hereditary susceptibility of endometriosis. Methods：Polymerase chain reaction（PCR） and DNA sequencing technique were used to detect the polymorphism of MIF gene promoter region-794CATT microsatellite in 43 cases with endometriosis（endometriosis group） and 44 cases without endometriosis（control group） of Han nationality in Guangdong,MIF genotyping was carried out. Results：The distribution frequencies of MIF gene promoter region-794CATT genotypes（5/5,5/6,5/7,6/6,7/7） in endometriosis group were 51.2%,11.6%,4.7%,30.2% and 2.3%,respectively;the distribution frequencies of MIF gene promoter region-794CATT genotypes（5/5,5/6,5/7,6/6,6/7,7/7） in control group were 25.0%,11.4%,2.3%,50.0%,4.5% and 6.8%,respectively,the distribution frequency of MIF gene promoter region-794CATT 5/5 genotype in endometriosis group was significantly higher than that in control group（P=0.015,OR=3.143,95%CI=1.281～7.713）;the distribution frequencies of MIF gene promoter region-794CATT alleles（5,6,7） in endometriosis group were 59.3%,36.0% and 4.7%,respectively;the distribution frequencies of MIF gene promoter region-794CATT alleles（5,6,7） in control group were 31.8%,58.0% and 10.2%,respectively;the distribution frequency of MIF gene promoter region-794CATT（5） allelotype in endometriosis group was significantly higher than that in control group（P=0.038,OR=3.122,95%CI=1.688～5.774）. Conclusion：The polymorphism of MIF gene promoter region-794CATT microsatellite is related to hereditary susceptibility of endometriosis among Han population in Guangdong,MIF-794CATT（5/5） genotype and MIF-794CATT（5） allelotype may be the susceptible genotypes.
出处
《中国妇幼保健》
CAS
北大核心
2011年第7期1086-1089,共4页
Maternal and Child Health Care of China
基金
深圳市科技局立项课题〔200902166〕
