姜黄素对肾小球系膜细胞过氧化物酶体增殖物激活受体γ基因表达的影响

Curcumin on Gene Expression of PPAR-γ in Glomerulus Mesangial Cells

目的:观察姜黄素对肾小球系膜细胞过氧化物酶体增殖物激活受体γ(PPAR-γ)mRNA基因表达水平的影响。方法:将高浓度葡萄糖液及不同浓度的姜黄素液与肾小球系膜细胞共孵育,用逆转录聚合酶链反应(RT-PCR)法,检测各组细胞PPAR-γ mRNA表达强度。结果:单纯高浓度葡萄糖组肾系膜细胞PPAR-γ mRNA表达量(0.394±0.026)显著低于正常对照组(0.995±0.016),P<0.01;当姜黄素的浓度达100mg/L时,其PPAR-γ mRNA积分吸光度比值为0.701±0.04,高于高浓度葡萄糖+姜黄素(25mg/L)组(0.541±0.018),显著高于高浓度葡萄糖+姜黄素(6.25mg/L)组(0.432±0.024),P<0.01。结论:姜黄素拮抗高浓度葡萄糖对肾系膜细胞PPAR-γ基因表达的抑制作用,上调PPAR-γ mRNA的表达水平。

Objective：Observe effects of Curcumin on gene expression of the peroxisome proliferator-activate receptors gamma（PPAR-γ） mRNA in glomerulus mesangial cells.Methods：High glucose concentration and Curcumin solutions of different concentrations were incubated with glomerulus mesangial cells by using reverse transcription-polymerase chain reaction（RT-PCR） to detect expression of PPAR-γmRNA of cells.Resuls： The expression of PPAR-γmRNA in glomerulus mesangial cells of the group of pure high glucose concentration was lower than the normal group（0.394±0.026 vs.0.995±0.016,P0.01）.When concentration of Curcumin reaches 100mg/L,the ratio of integral optical density of its PPAR-γmRNA was,higher than that of Gurcunin 25 mg/L group（0.701±0.04 vs.0.541±0.018,P0.01）and it was significant higher than that of Curcumin 6.25 mg/L group（0.432±0.024,P0.01）.Conclusions： Curcumin will antagonize inhibition effect of high glucose concentration on gene expression of PPAR-γ in glomerulus mesangial cells,and increase the expression level of PPAR-γmRNA.