摘要
目的:观察白介素6(IL-6)抗原及IL-6抗体对2型糖尿病(T2DM)大鼠的影响,探讨IL-6等炎症因子对糖尿病胰岛素抵抗的作用。方法:36只SD T2DM大鼠,随机分为4组:糖尿病IL-6抗原组(A组),糖尿病IL-6抗体组(B组),糖尿病对照组(C组),健康对照组(N组)。测定血糖、血清胰岛素、血脂、血游离脂肪酸和肝脏胰岛素受体。结果:脂肪乳灌胃(高糖高脂饮食)7周加小剂量1次尾静脉注射链脲佐菌素(STZ)可以成功诱导SD大鼠的T2DM模型,糖尿病组较正常对照组出现明显的高胰岛素血症、胰岛素抵抗、胰岛素敏感性降低和高脂血症;IL-6抗体干预可以降低T2DM大鼠的胰岛素水平,减轻其胰岛素抵抗。结论:IL-6抗体能够改善T2DM大鼠的胰岛素抵抗状态。
Objective：To observe the influence of interleukin-6（IL-6） antigen and IL-6 antibody on type 2 diabetes mellitus rats,and to study the important role of IL-6 and other inflammation factors in the pathogenesis of diabetes.Methods： A total of 36 the type 2 diabetes mellitus SD rats were divided into four groups randomly：the diabetes mellitus IL-6 antigen group（Group A）,diabetes mellitus IL-6 antibody group（Group B）,diabetes mellitus control group（Group C）,and normal control group（Group N）.The serum insulin,lipid,free fatty acid and the insulin receptor on the liver were measured.Results： Using intragastric administration with fat milk（high-glucose-high-fat diet） plus low dose STZ by vena caudalis,the type 2 diabetes mellitus SD rat model could be induced successfully.Contrast to control group,the serum insulin levels of diabetes mellitus Group A,B and C were significant higher,the insulin sensitivity decreased and the insulin resistant increased.And IL-6 antibody could decrease insulin level and ease insulin resistant in type 2 diabetes mellitus rat.Conclusions： IL-6 antibody could ease insulin resistant in type 2 diabetes mellitus rat.
出处
《中国临床医学》
2011年第1期32-34,共3页
Chinese Journal of Clinical Medicine
基金
天津市卫生局科技基金计划项目(编号:05KYZ65)
