海燕体壁胶原蛋白提取工艺的初步研究

Extraction of collagen from body wall of star fish Asterina pectinifera

采用两种不同的方法从海燕Asterina pectinifera体壁中提取出酸溶性胶原蛋白(ASC)和胃蛋白酶促溶性胶原蛋白(PSC)。结果表明:ASC的最佳反应条件是,温度为10℃,提取液种类为乙酸-乙酸钠溶液,提取液浓度为0.5 mol/L,提取时间为72 h,ASC得率为7.93%;PSC的最佳反应条件是,温度为17℃、胃蛋白酶酶加量为4%、提取时间为96 h,PSC得率为49.61%。经紫外光谱和氨基酸组成分析,结果表明,ASC和PSC均为典型的Ⅰ型胶原蛋白。

Acid-soluble collagen（ASC） and pepsin-solubilized collagen（PSC） were extracted from body wall of star fish Asterina pectinifera by two different methods.The optimum extraction of ASC with the yield of 7.93% were found at hydrolyzing temperature of 10 ℃,hydrolyzing time of 72 h and the concentration of 0.5 mol/L acetic acid-sodium acetate.The optimum conditions for the extraction of PSC with yield of 49.61% were found as follows：hydrolyzing temperature of 17 ℃,hydrolyzing time of 96 h,and the pepsin dose of 4%.The UV spectra and Amino Acid Analysis revealed that ASC and PSC from body wall of the star fish were classified as type I collagen.