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Ezrin对人肺癌细胞株A549侵袭转移能力的影响及机制 被引量:2

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摘要 目的观察Ezrin对人肺癌细胞株A549侵袭转移能力的影响,并探讨其机制。方法用细胞转染方法将Ezrin特异性siRNA(观察组)和对照siRNA(对照组)转入A549后,采用Western blot法检测A549中Ezrin蛋白表达情况,采用Transwell实验检测穿过膜的A549细胞数量,采用RT-PCR方法检测A549中MMP-2、MMP-9、u-PA、c-Fos、c-Jun和NF-κB mRNA的表达。结果与对照组相比,siRNA干扰Ezrin基因后,A549细胞穿过膜的细胞数量明显减少(P均<0.01),u-PA、c-Fos、c-Jun和NF-κB的表达明显降低(P均<0.01)。结论 Ezrin通过诱导c-Fos、c-Jun和NF-κB三种转录因子活性,进而通过u-PA促进A549细胞的侵袭和转移能力。
作者 王立文
出处 《山东医药》 CAS 北大核心 2011年第6期80-81,共2页 Shandong Medical Journal
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参考文献8

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