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良性前列腺增生上皮对间质细胞14-3-3β基因表达的影响

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摘要 目的观察良性前列腺增生(BPH)上皮对间质细胞14-3-3β基因表达的影响。方法分离BPH组织的间质细胞与上皮细胞,构建上皮、间质细胞共培养模型。分别提取单独(对照组)、与上皮细胞共培养间质细胞(观察组)的mRNA,采用RT-PCR法检测14-3-3β mRNA。结果观察组14-3-3β mRNA的表达量为0.75±0.36,明显低于对照组的0.88±0.37,P<0.01。结论 BPH上皮细胞可抑制间质细胞14-3-3β基因的表达。
出处 《山东医药》 CAS 北大核心 2011年第6期93-94,共2页 Shandong Medical Journal
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