摘要
提出了高效液相色谱法测定乳及乳制品中4种人工合成甜味剂安赛蜜、糖精钠、阿斯巴甜和纽甜含量的方法。样品加入亚铁氰化钾和乙酸锌使蛋白质沉淀析出后,以Diamonsil-C18色谱柱(250 mm×4.6 mm,5μm)为固定相,0.05 mol.L-1磷酸二氢钾溶液和乙腈为流动相梯度洗脱,用二极管阵列检测器于230 nm波长处检测安赛蜜,210 nm波长处检测糖精钠、阿斯巴甜和纽甜。安赛蜜、糖精钠、阿斯巴甜和纽甜的方法检出限(3S/N)分别为1.0,0.5,1.5,2.5μg.g-1。以空白乳粉样品为基体作加标回收试验,测得回收率在90.0%～103.3%之间,相对标准偏差(n=8)在2.1%～5.8%之间。
HPLC was applied to the determination of 4 artificial edulcorators,i.e.,acesulfame,sodium saccharin,aspartame and neotame in milk and its products.The sample was added with KFe（CN）3 and Zn（OAc）2 to precipitate protein,and the edulcorator was separated by gradient elution with a mixed solution of 0.05 mol·L-1 KH2PO4 and acetonitrile in different ratios on Diamonsil-C18 column（250 mm×4.6 mm,5 μm）.Diode array detector was used for the determination of acesulfame at 230 nm and for sodium saccharin,aspartame and neotame at 210 nm.Values of detection limits（3S/N） of the method for the 4 edulcorators were found to be 1.0,0.5,1.5 and 2.5 μg·g-1 respectively.Using blank sample of milk powder as matrix,recovery test was made by standard addition method,values of recovery were found in the range of 90.0%-103.3% and RSD′s（n=8） found were ranged from 2.1% to 5.8%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2011年第2期150-153,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
