摘要
提出了离子色谱法测定液体乳中硫氰酸盐的方法。鲜奶或酸奶样品经乙腈提取和C18固相萃取柱净化后,再经Ion Pac AG11-HG保护柱及Dionex ICS-3000分离柱分离,由70 mmol.L-1氢氧化钾溶液洗脱,利用电导检测器检测。硫氰酸盐的质量浓度在0.20～10.0 mg.L-1范围内呈线性,方法的检出限(3S/N)为0.08 mg.L-1。方法用于鲜奶和酸奶样品分析,回收率在92.5%～104.5%之间,相对标准偏差(n=6)小于4.5%。
Ion chromatography was applied to the determination of thiocyanate in liquid dairy product.The samples of fresh milk or sour milk were extracted with acetonitrile and purified on C18 SPE column.The protective column（Ion Pac AG11-HG） and separation column（Dionex ICS-3000） were used for separation,with 70 mmol·L-1 KOH solution as mobile phase,and conductivity detector was used in the determination.Linearity range was found between 0.20-10.0 mg·L-1,and detection limit（3S/N） was found to be 0.08 mg·L-1.The proposed method was applied to the analysis of fresh milk and sour milk,giving values of recovery in the range of 92.5%-104.5% with RSD′s（n=6）less than 4.5%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2011年第2期211-212,215,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
关键词
离子色谱法
电导检测器
鲜奶
酸奶
硫氰酸盐
Ion-chromatography
Conductivity detector
Fresh milk
Sour milk
Thiocyanate
