枸杞髓组织离体培养及高频率植株再生的研究

In vitro culture of Pith tissue and high-frequency plant regeneration of Lycium barbarum L.

枸杞髓组织在４种ＭＳ培养基上都能诱导出愈伤组织，诱导率５３．７％～１００％。在培养基ＭＳ＋６－ＢＡ０．１ｍｇ／Ｌ＋ＮＡＡ０．５ｍｇ／Ｌ获得的愈伤组织，呈颗粒状，分散性能好，胚性细胞多．将其转移到ＭＳ＋６－ＢＡ０．５ｍｍ／Ｌ＋ＮＡＡ０．０１ｍｇ／Ｌ的分化培养基上获得大量绿色小芽，小芽在ＭＳ＋６－ＢＡ０．２ｍｇ／Ｌ的培养基上得到快速繁殖，繁殖系数５０～１５０株／芽·月。丛生芽在ＭＳ＋ＮＡＡ０．

A study on Pith tissue culture on MS medium with different hormone, the induction ratew 53.7% ~100%. Among which the calli induced on medium MS+6-BA 0.1mg / L+NAA 0.5mg / L were smallest in Particle size and had good dispersion ability with a large quantity of embryonic Cells The cant were moved onto Ms+6-BA 0.5 mg / L+NAA 0.01mg / L differentiation medium and a large quantity of embryonic Plants were obtained The embryonic plants were reproduced rapidly after they are removed onto medium MS+6-BA 0.2mg / L with the reproductionrate of 50~150 clusters shoot / bud.month. Complete plants were induced on MS+NAA 0.2mg / Lmedium