影响木薯次生胚状体发生及植株再生因素的研究

Factors influencing the development of secondery embryoids and plant regeneration on Cassava(Manihot esculenta Crantz)

研究了在外植体的不同发育阶段中，碳源以及不同的生长激素配比对木薯次生胚状体诱导及植株再生的影响。结果表明：以固体成熟培养基上生长１５ｄ的胚状体子叶为外植体，次生胚状体的产量最高，达２９．３个成熟胚状体／１个外植体。在次生胚状体的诱导阶段，以麦芽糖（４０ｇ／Ｌ）代替蔗糖作碳源，能同时提高次生胚状体的产量（３２．５个胚次体／１个外植体）及植株再生频率（７４．３％）。２，４－Ｄ与ＰＰ３３３；（０．１ｍｇ／Ｌ）配合能提高植株再生频率到７７．６％。２，４－Ｄ与ＢＡＰ（２ｍｇ／Ｌ）或激动素（２．０ｍｇ／Ｌ）配合则大大降低了胚状体诱导及植株再生频率。

During the different growth stages of Cassava explants, the effects of carbon sources andgrowth regulators with different concentrations and combinations on the development of secondaryembryoids and plant regeneration were investigated with an approach based on the cyclicembryogenesis liquid culture system. The highest embryoid production i. e., 29.3 maturationembryoids per explant, was obtained from initial embryoids being maturated on the solid medium(MS medium supplemented with 0.1 mg / L BAP) for 15 d as explants. When maltose (40 g / L) wassubstituted for sucrose as carbon source in the culture medium, there was an increasing Potential bothin the efficiency of embryoid development (32.5 mature embryoids Per explant) as well as the frequency of plant regeneration (74.3%). An increase of 38.5% to 77.6% regeneration frequency was demonstrated when 2,4-D and PP,3,(0. 1 mg / L ) were contained in the culture in the culture medium. However, with a combination of 2,4-D and either BAP (2mg / L) or kinetin (2.0 mg / L), a considerablereduction both in embryoid induction and frequency of plant regeneration was observed.