短叶省藤种质离体培养保存研究

In vitro conservation of germplasm of Calamus egregius

本文对短叶省藤种质的离体培养保存进行了探索性研究。结果表明：种质以丛芽形式，采用ＲＥＯ保存培养基，在温度１２．５℃和光照３００１Ｘ×１２ｈ／ｄ的保存条件下，可安全保存１８个月以上，保存率＞２５％，丛芽平均高生长小于２．２ｃｍ。种质保存一年后，转接至增殖或生根培养基，仍可诱导增殖和生根．结果表明离体保存具一定可行性，其保存模式可预定为：增殖培养２个月＋离体保存１８个月的循环模式．

This paper deals with in vitro conservation of germplasm of Calamus egregius BurretStored on REO medium with temperature of 12.5 + 0.5 C and illumination of 300 + 50 lx 12hours / day, clumps with 1~3 buds can be Preserved safely for 18 months without transfer, and thesurvival rate>25 %, the height increment <2.2 cm After one year's conservation, when transfer-red onto Proliferation or rooting media, the germplasm can still be induced to multiply or to root. InVitro conservation Procedure of germplasm of Calamus egregius could be scheduled as a circulationof 2 months' Proliferation culture + 18 months' conservation culture.