LI-cadherin在胃癌患者血清及组织中表达的研究

Expression of LI-cadherin in serum and tissue of patients with gastric cancer

目的研究胃癌血清及组织中肝肠钙黏蛋白(LI-cadherin)的表达水平及其临床意义。方法应用酶联免疫吸附法(ELISA)技术测定48例胃癌患者血清LI-cadherin的浓度,并与29例正常对照血清做比较;同时用SP免疫组化技术半定量测定此48例胃癌患者癌灶中LI-cadherin表达,与血清LI-cadherin比对。结果胃癌患者及正常对照血清中均能检测出LI-cadherin。胃癌组血清LI-cadherin表达水平明显高于对照组,具有显著性差异(P<0.001);LI-cadherin表达水平与肿瘤分化程度(P<0.01),Lauren's分型(P<0.05)密切相关;LI-cadherin血清含量可以反映其在肿瘤组织中的表达,二者水平呈正相关。结论血清LI-cadherin含量可以反映胃癌的临床病理情况,在诊断和评价胃癌患者病情方面,是一个价值的肿瘤标记物。

Objective To evaluate the clinical significance and expression of serum LI-cadherin and tissue LI-cadherin in gastric cancer.Methods Serum level of LI-cadherin was measured with enzyme-linked immunosorbent assay（ELISA） in 48 patients with gastric cancer and 29 controls.At the same time semi-quantitative immunohistochemistry was used to detect the expression of LI-cadherin in tissue and compared with the serum concentration.Results The expression of LI-cadherin was detected in both gastric cancer patients and normal control.The serum level of LI-cadherin in gastric cancer patients was significantly higher than that in controls（P 0.001）.LI-cadherin expression was significantly correlated with tumor differentiation（P 0.01）,and Lauren＇s classification（P 0.05）.Serum LI-cadherin levels and LI-cadherin expression in tumor tissues were positively correlated.Conclusion Serum levels of LI-cadherin can reflect the progress of clinical pathology and tumor metastasis.It may be a valuable marker for the diagnosis and evaluation of gastric cancer. 【