乙型肝炎病毒全基因序列的测定

Sequencing of the Whole Gene of Hepatitis B Virus

测定７ 例慢性 Ｈ Ｂ Ｖ 携带者 Ｈ Ｂ Ｖ 基因组全序列，经同源性比较，确定基因型。２ 例基因型为 Ｂ 型，余均为 Ｃ 型；血清型ａｄｒ ４ 例，ａｄｗ ３ 例。各序列间 Ｘ 基因差异最大。未见 Ａ１８９６ 、 Ｔ１７６２ Ａ１７６４等重要位点的变异。结合已有的２ 株 Ｈ Ｂ Ｖ 中国流行株全基因序列，初步建立以中国流行株序列为基础的 Ｈ Ｂ Ｖ 标准序列，该标准序列与国外标准序列仅有２２

Seven cases of chronic hepatitis B virus carriers were included for sequencing of whole gene sequences of hepatitis B virus (HBV). The serotype of 4 strains of HBV were adr, and 3 strains were adw. Two strains were classified to genotype B, and the other 5 strains to genotype C. No significant mutations, such as A 1896 , T 1762 A 1764 were present. With other reported 2 complete sequences of HBV strains prevailing in China's Mainland, 7 strains of HBV whole sequences of genotype C from China's Mainland were analyzed to produce the consensus sequence of HBV of China. Comparing of the consensus sequence with that from Genbank, there were only 22 sites with different nucleotides.