艾灸腹部募穴调整大鼠肠道菌群失调的实验研究(英文)

Moxibustion at Front-Mu Point of Abdomen for Intestinal Dysbacteriosis in Rats

目的:观察艾灸关元、天枢对实验性大鼠肠道菌群失调的影响。方法:将50只清洁级Wistar大鼠随机分为正常组(Normal group)、模型组(Model group)、药物组(Medical group)、关元组(CV4group)、天枢组(ST25group),每组10只。用大量盐酸林可霉素灌胃造模,造模成功后,分别进行药物治疗和艾灸治疗。1个疗程后,采取各组大鼠新鲜粪便0.1g,应用双歧杆菌(Bifidobacterium Species,BS)、乳酸杆菌(Lactobacillus,LBS)、肠杆菌(Enterobacteriaceae,EB)、肠球菌(Enterococcus,EC)选择性培养基进行细菌培养,生化鉴定管和比浊法检测不同菌落生长情况和各组菌落数量。结果:艾灸关元穴使BS、LBS数量有所增加;艾灸天枢穴使EB、EC数量有所增加。结论:艾灸不同部位的募穴可以选择性调整肠道优势益生菌群,从而治疗肠道菌群失调症。

Objective：To study the moxibustion at Guanyuan（CV 4）and Tianshu（ST 25）on intestinal dvsbacteriosis in rats.Methods：Fifty Wistar rats,clearing grade,were randomly divided into 5 groups,a normal group,a model group,a medical group,and a Guanyuan（CV 4）group and a Tianshu（ST 25）group,with 10 rats in each group.The rats were treated with Lincomycin Hydrochloride via intragastric administration for developing models.The model rats were treated with medicine and moxibustion respectively.0.1 g fresh rat feces in each group were cultured on the selective culture medium of bifidobacterium species（BS）,lactobacillus（LBS）,enterobactefiaceae（EB）and enterococcus（EC）.The growth and quantity of the bacterial colony were tested by biochemical identification tubes and turbidimetry.Results：Moxibustion at Guanyuan（CV 4）increased BS and LBS while moxibustion at Tianshu（ST 25）improved EB and EC.Conclusion：Moxibustion at Front-Mu points Of different body parts selectively regulated advantaged probiotics for treating intestinal dysbactenosis.