红曲霉粗多糖的提取及含量测定

Extraction,Isolation and Determination of Polysaccharides from Monascus

目的:从红曲霉发酵液和菌丝体中提取粗多糖并测定多糖含量。方法:将红曲霉发酵液和菌丝体用水提醇沉法提取粗多糖,用苯酚-硫酸法测定红曲霉多糖的含量。结果:红曲霉粗多糖中多糖含量为62.87%,平均回收率为99.35%,RSD=1.44%。结论:本实验方法可以提取出红曲霉多糖,多糖含量测定准确,苯酚-硫酸法可作为红曲霉多糖含量的测定方法。

Objective： To extract the polysaccharide in the fermentation broth and mycelium of Monascus and determine its content. Methods：Crude polysaccharide of Monascus was extracted by water extraction and alcohol precipitation method,and the content was determined by the phenol-sulfuric acid method. Results：The polysaccharide content was 62.87% with an average recovery of 99.35% and RSD of 1.44%. Conclusion：The polysaccharides can be extracted from Monascus by the mentioned methods. The determination for content of polysaccharide was proved accurate,and the phenol-sulfuric acid method can be used to determine the content of polysaccharide.