甲基苯丙胺中毒小鼠脑组织神经元超微结构变化的研究

Ultrastructural Changes in Mice Brain Neurons after Methamphetamine Intoxication

目的:通过观察甲基苯丙胺中毒后小鼠脑组织超微结构的改变,探讨脑组织超微结构改变与甲基苯丙胺神经毒性机制的关系。方法:将40只小鼠随机分为对照组和3组实验组(A,B,C)。A组给予MA(20mg/kg,ip,single)、B组给予MA(20mg/kg,8am,8pm,ip×2d)、C组给予MA(20mg/kg,8am,8pm,ip×4d),对照组给予等量生理盐水。用电镜观察前额叶皮质、海马、纹状体三个部位组织神经元胞体超微结构改变,并与空白对照组比较,结果进行统计学处理。结果:给予MA后,小鼠各脑区神经元胞体出现神经元固缩、变性、凋亡、坏死等超微病变。结论:MA可诱导神经细胞发生神经元固缩、变性、凋亡、坏死等超微病变,其变化程度随时间和药物蓄积有逐渐增加的趋势。

Objective：To investigate the relationship between the brain organization ultrastructural changes and methyl benzene propylamine nerve toxicity mechanism.by detecting the ultrastructural changes of brain tissue in methamphetamine poisoning mice.Methods：A total of 40 mice were divided into control group and experimental groups（A,B,C）,with 10 mouses in each one.Mouses in group A were injected with MA（20 mg·kg-1,ip,single）;Mouses in group B were injected with MA（20 mg·kg-1,8 am 8 pm,ip ×2d）;Mouses in group C were injected with MA（20 mg·kg-1,8 am 8 pm,ip ×4 d）;while the mice of the control group received normal saline.The changes of prefrontal cortex,hippocampus,basal ganglia organization of ultrastructural were detected by electron microscopy.Results：There were condensation,degeneration,apoptosis,necrosis,ultrastructural alteration in brain neurons after the mice were given MA.Conclusion：MA can induce the brain neurons generate cellular swelling,apoptosis,necrosis and cell pyknosis,the severity level of those pathological changes were increased with the longer of the time and the increased of injection times with MA.