小檗碱对动脉粥样硬化性损伤的保护作用

Protective Effect of Berberine on Atherosclerotic Injury

目的:探讨小檗碱对动脉粥样硬化损伤的保护作用。方法:将56只大鼠,随机分为对照组(C),高脂饮食假手术组(HD),肾动脉狭窄组(RAS),肾动脉狭窄高脂饮食组(HD+RAS)。C及RAS组给予正常饮食,HD及HD+RAS组给予高脂饮食,RAS及HD+RAS组采用固定内径银夹夹左肾动脉。饲养12周,每组处死6只进行检测,剩余除C外每日灌胃小檗碱(150mg/kg),C予以等量生理盐水灌服,持续4周,四周后处死进行相同检测,用药前后结果进行对比评价小檗碱功能。结果:与C相比,HD,RAS,HD+RAS组肌酐显著升高(P<0.05),HD及HD+RAS组胆固醇明显升高(P<0.05),超氧化物歧化酶(SOD)在HD,RAS及HD+RAS组显著降低(P<0.05),丙二醛(MDA)在HD,RAS及HD+RAS组显著增高(P<0.05),血管内皮生长因子VEGF在HC,RAS及HD+RAS组表达量升高。给予小檗碱灌胃处理后,检测指标各组间无统计学差异(P>0.05)。结论:小檗碱对动脉粥样硬化性损伤具有良好的保护作用。

Objective：To investigate the protect effect of Berberine on atherosclerotic injury.Methods：Fifty-six rats were randomly divided into control group（C）,high-fat diet group（HD）,renal artery stenosis group（RAS）,high-fat diet with renal artery stenosis group（HD＋RAS）,fourteen in each group.C and RAS were given proper diet,HD and HD＋RAS were given high fat diet.RAS and HD＋RAS were clamped by sliver clip with a fixed diameter on left renal artery.12 weeks later,executed six of every group for testing,the left were given Berberine（150mg/kg） every day except C,C were given saline.4 weeks later,the left rats were executed for the same testing.Results：Compared with C,Creatinine was significantly higher in other three groups（p〈0.05）.Cholesterol was significantly higher in HD and HD＋RAS groups（p0.05）.SOD was Significantly higher while MDA was Significantly lower in HD,RAS and HD＋RAS group.The ex-pression of VEGF was significantly higher in HD,RAS and HD＋RAS group.After given berberine,the testing results had no significant difference in different groups.Conclusions：Berberine has good protection effect on atherosclerotic injury.