电磁辐射对大鼠内皮祖细胞和肾脏组织学的影响

The Effects of Electromagnetic Radiation on Endothelial ProgenitorCells and Renal Histology

目的:研究电磁辐射对体外培养骨髓来源的内皮祖细胞(EPCs)增殖、迁移、黏附能力的影响,并探讨其与肾脏疾病的可能关系。方法:密度梯度离心法获取大鼠骨髓单个核细胞(MNCs),接种至纤维连接素包被的培养板上,培养6d后进行免疫细胞化学和免疫荧光鉴定EPCs。采用MTT比色法、Transwell小室和黏附能力测定实验,观察平均功率密度为65mW/cm2,时间20min的电磁辐射对EPCs的增殖、迁移、黏附能力的影响;同等剂量全身照射大鼠,光镜和透射电镜观察大鼠肾脏组织学和超微结构的变化。结果:从大鼠骨髓能成功分离培养获得EPCs。EPCs的增殖、迁移、黏附能力较对照组显著下降;大鼠接受全身照射后各时相点无明显组织学改变,但超微结构显示在照射后3h后开始出现肾小球毛细血管袢足突肿胀,12h后出现部分足突融合。结论:电磁辐射导致EPCs生物功能显著减弱,肾小球超微结构改变,电磁辐射可能与起肾脏疾病的发生有关。

Objective：To investigate the effects of electromagnetic radiation on proliferation,migration,adhesion of endothelial progenitor cells（EPCs） cultured in vitro derived from rats bow marrow,and to investigate the relationship between electromagnetic radia-tion and kidney disease.Methods：Mononuclear cells（MNCs） were obtained from rats＇ bone marrow by density gradient centrifugation,cultured with EGM-2 complete medium on plate coated by fibronectin.After 6 days,the cells were identified by immunocytochemistry and immunofluorescence.The effects of electromagnetic radiation with 65 mW/cm2 for 20minutes on EPCs proliferation,migration,adhesion were detected by MTT colorimetric method,Transwell assay and adherence ability tests,and rats＇ kidney histological and ultra-structural changes of irradiating rats was detected.Results：EPCs could be obtained successfully by culture the MNCs form rats bone marrow.Compared with the control group,EPCs proliferation,migration and adhesion ability decreased remarkably.There was no obvi-ous histological change when the rats received the irradiation at any time point.But the ultrastructure showed that there were podocytes swelling after irradiation of 3 hours and fusion after 12 hours in glomeruli capillary loops.Conclusions：Electromagnetic radiation can remarkably depress EPCs biological function and change glomeruli ultrastructure.Electromagnetic radiation probably caused the occur-rence of kidney diseases.