白介素10和白介素17基因启动子区多态性位点与儿童哮喘的相关性研究

Association between Polymorphisms in Promoter Region of Interleukin-10 and Interleukin-17 Gene and Childhood Asthma

目的:探讨IL-10基因启动子区-627A/C和IL-17基因启动子-152A/G位点多态性与儿童哮喘发生的相关性。方法:采用聚合酶链反应-限制性片段长度多态性分析(PCR-PFLP)方法检测186名哮喘儿童、198名健康儿童各个多态性位点的基因型,采用SPSS13.0进行统计学分析。结果:IL-17基因-152A/G位点的基因型及等位基因频率分布在哮喘组与正常对照组均存在显著性差异(p<0.05),哮喘组-152A/G位点等位基因A频率显著高于正常对照组(x2=6.077,p=0.014,OR=1.430,95%CI=1.076-1.902)。结论:IL-17基因-152A/G位点可能与儿童哮喘的发病存在关系,其中A等位基因可能是易感基因,携带A的个体可能更易患有哮喘。

Objective：To investigate the relationship between the-627A/C of interleukin-10 and-152A/G of interleukin-17 gene polymorphisms and Childhood asthma.Methods：Genomic DNA was isolated from the venous blood leukocytes of 186 unrelated patients with Childhood asthma and 198 healthy unrelated individuals（control group）.Polymorphisms-627A/C and-152A/G were genotyped by PCR-restriction fragment length polymorphisms（PCR-RFLP）.Genotype and allele frequencies were analyzed by SPSS13.0 software.Results：There were significant differences for both allele and genotype frequencies of-152A/G of IL-17 gene between the Childhood asthma and control group.The allele A of-152A/G in Childhood asthma group was significantly higher than that in control group（p〈0.05）,Conclusion：There is association between-152A/G of IL-17 gene and Childhood asthma.The individuals with A allele of-152A/G are susceptible to Childhood asthma.