狂犬病毒街毒株糖蛋白基因克隆、测序与表达

Cloning, Sequencing and Expression of Glycoprotein Gene of Chinese Street Strain(SBD07) of Rabies Virus

经实验研究证明狂犬病毒街毒株SBD0 7株具有较好的免疫原性后 ,通过RT PCR法 ,克隆了该毒株的糖蛋白 (G)基因 ,并进行了全序列测定。结果表明该株狂犬病毒G基因与疫苗株 (aG株 )的核酸及蛋白同源性分别为 85 8%和 88 5 %。以痘苗病毒为载体成功地表达了该基因 ,经小鼠实验表明能诱生较高滴度的狂犬病毒中和抗体 。

After the good immunogenicity of Chinese street strain(SBD07) of rabies virus had been proved, the glycoprotein gene of the strain was cloned and sequenced by RT PCR. The result showed that nucleotide and protein homologies between the genes of SBD07 and vaccine strain(aG) were 85.8% and 88.5% respectively. The glycoprotein gene of SBD07 was successfully expressed using vaccinia virus as vector. The expressed product could induce high level of neutralizing antibodies to rabies virus in mice and protect them from lethal challenge of rabies virus CVS strain intramuscularly.