摘要
分别以大麦花药、幼穗、幼胚为外植体诱导愈伤组织，比较了它们的出愈率和愈伤组织经部分去壁ＰＥＧ＋电击法转化后的存活率、转化率。结果表明：以“舟麦２号”幼穗愈伤组织作为遗传转化的受体最为合适。
Calli were induced from anthers,immature inflorecences and immature embryoes of barley ( Hordeum vulgare L.). Their differentiation frequency,survival rate and transformation rate were studied. The results showed that calli derived from immature inflorecences of 'Zhoushan No. 2' were the most suitable receptor for our foreign gene transfer system.
出处
《浙江师大学报（自然科学版）》
CAS
1999年第1期62-66,共5页
Journal of Zhejiang Normal University(Natoral Sciences)
关键词
大麦
愈伤组织
遗传转化
PEG
电击法
barley ( Hordeum vulgare L.)
callus
genetic transformation
PEG + electroporation
