用电化学方法和光度法研究手性乙二胺合钴(Ⅲ)配合物与DNA的作用(英文)

STUDY OF BINDING MODES OF λ\| AND δ\|〔Co(en)\-3〕\+ 3+ W ITH CALF THYMUS DNA

采用电化学法和光度法研究了手性乙二胺合钴（Ⅲ）配合物与 Ｄ Ｎ Ａ 的作用。结果表明，两种旋光对映体与 Ｄ Ｎ Ａ 发生作用时具有相同的电化学行为和相同的紫外可见光谱。平衡透析实验结果也证明，两种旋光对映体与 Ｄ Ｎ Ａ 作用时，不存在立体选择性，即插入作用，这可能与配体分子结构的非平面性有关，两种对映体与 Ｄ Ｎ Ａ作用时，主要存在静电吸引作用。

The binding of enantiomers of 〔Co(en)\-3〕\+ 3+ (en=ethylenediamine) to calf thymus DNA is investigated by electro chemical and spectroscopic methods. Both isomers show absorption hypochromicity and the same CV behavior associated with binding to B\|form DNA. CD spectra resu lt shows no enantioselectivity for both isomers binding to DNA. All of this evid ence suggests that both isomers bind to DNA by electrostatic mode.