摘要
目的:利用pTet-off可诱导表达系统将NAIF1重组质粒导入食管癌EC9706中进行生物学行为影响研究。方法:构建pTRE2-NAIF1质粒,与PTK-Hyg质粒共转染入可诱导表达细胞系TF93(由EC9706构建而得),经筛选并鉴定获得稳定可诱导表达克隆。以此克隆采用MTT法检测细胞增殖、流式细胞术(FCM)检测细胞周期、Transwell小室法检测细胞迁移和检测细胞黏附能力的改变。结果:经筛选并鉴定获得了稳定的可诱导表达TF93-pTRE2-NAIF1克隆。NAIF1在体外可以抑制EC9706细胞增殖、导致G0/G1期细胞阻滞、抑制其迁移和黏附能力。结论:NAIF1能抑制食管癌细胞系EC9706的恶性生物学行为,为食管癌的基因治疗提供了可能的新靶点。
AIM： To investigate the effects of NAIF1 on biological behavior of esophageal carcinoma cell line EC9706 by using pTet-off system. METHODS： Constructe the pTRE2-NAIFI plasmid and cotransfected with PTK-Hyg in human inducable esophageal carcinoma cell line TF93 （derive from EC9706 cell line）. After Hygromycin B selection and RT-PCR, Western blot identification we got stable inducable cell clone TF93-pTRE2-NAIF1. By using this clone, we used MTT to test the proliferation rate, flow cytometry to test the cell cycle, Transwell chamber to test the migration ability and tested the adhesion ability of EC9706 under the effect of NAIFI. RESULTS： First we got the stable inducable cell clone TF93-pTRE2-NAIFl, then we found NAIFI could inhibit the cell proliferation, cause G0/G1 cell arrest and inhibit the migration and adhesion ability of EC9706. CONCLUSION： NAIFI can inhibit the malignant phenotype of esophageal carcinoma cell line EC9706.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2011年第2期162-165,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家重大基础研究发展计划(973)资助项目(2007CB914700)
